GlutaredoxinV1, Main, Exploration, bibRecord, 000478

Acid sphingomyelinase as target of Lycium Chinense: promising new action for cell health.

Identifieur interne : 000478 ( Main/Exploration ); précédent : 000477; suivant : 000479

Acid sphingomyelinase as target of Lycium Chinense: promising new action for cell health.

Auteurs : Maria Rachele Ceccarini [Italie] ; Michela Codini [Italie] ; Samuela Cataldi [Italie] ; Samuele Vannini [Italie] ; Andrea Lazzarini [Italie] ; Alessandro Floridi [Italie] ; Massimo Moretti [Italie] ; Milena Villarini [Italie] ; Bernard Fioretti [Italie] ; Tommaso Beccari [Italie] ; Elisabetta Albi [Italie]

Source :

Lipids in health and disease [ 1476-511X ] ; 2016.

RBID : pubmed:27756324

Descripteurs français

KwdFr :
- Antioxydants (administration et posologie), Antioxydants (composition chimique), Cellules HepG2 (MeSH), Extraits de plantes (administration et posologie), Extraits de plantes (composition chimique), Fruit (composition chimique), Humains (MeSH), Lycium (composition chimique), Médecine traditionnelle chinoise (MeSH), Régulation de l'expression des gènes (effets des médicaments et des substances chimiques), Sphingomyeline phosphodiesterase (biosynthèse), Sphingomyéline (métabolisme), Survie cellulaire (effets des médicaments et des substances chimiques).
MESH :
- administration et posologie : Antioxydants, Extraits de plantes.
- biosynthèse : Sphingomyeline phosphodiesterase.
- composition chimique : Antioxydants, Extraits de plantes, Fruit, Lycium.
- effets des médicaments et des substances chimiques : Régulation de l'expression des gènes, Survie cellulaire.
- métabolisme : Sphingomyéline.
- Cellules HepG2, Humains, Médecine traditionnelle chinoise.

English descriptors

KwdEn :
- Antioxidants (administration & dosage), Antioxidants (chemistry), Cell Survival (drug effects), Fruit (chemistry), Gene Expression Regulation (drug effects), Hep G2 Cells (MeSH), Humans (MeSH), Lycium (chemistry), Medicine, Chinese Traditional (MeSH), Plant Extracts (administration & dosage), Plant Extracts (chemistry), Sphingomyelin Phosphodiesterase (biosynthesis), Sphingomyelins (metabolism).
MESH :
- chemical , administration & dosage : Antioxidants, Plant Extracts.
- chemical , biosynthesis : Sphingomyelin Phosphodiesterase.
- chemical , chemistry : Antioxidants, Plant Extracts.
- chemistry : Fruit, Lycium.
- drug effects : Cell Survival, Gene Expression Regulation.
- chemical , metabolism : Sphingomyelins.
- Hep G2 Cells, Humans, Medicine, Chinese Traditional.

Abstract

BACKGROUND

Sphingomyelin plays very important roles in cell function under physiological and pathological conditions. Physical and chemical stimuli produce reactive oxygen species that stimulate acid sphingomyelinase to induce apoptosis. Antioxidant plants of the traditional Chinese Pharmacopoeia, such as Lycium Barbarum and Lycium Chinense, have become increasingly popular in Western countries. We investigated the effects of Lycium Chinense on acid sphingomyelinase and sphingomyelin species in relation to gene expression.

METHODS

We prepared Lycium Chinense berry extracts and evaluated their antioxidant properties. Increasing amount of extracts was used to test cytotoxic and genotoxic effect on HepG2 cells. Gene expression, protein amount and enzyme activity of acid sphingomyelinase were tested by RT-PCR, immunoblotting and enzymatic activity assay, respectively. Sphingomyelin species were analyzed by UFLC MS/MS. A panel of 96 genes involved in oxidative stress, proliferation, apoptosis and cancer was used to test the effect of LC on gene expression. GLRX2, RNF7, and PTGS1 proteins were analyzed by immunoblotting.

RESULTS

We showed that Lycium Chinense berries have high antioxidant properties, have an IC50value of 9.55 mg/mL, do not induce genotoxic effect and maintain high level of cell viability. The berry extracts inhibit acid sphingomyelinase activity and increase both very long fatty acid sphingomyelin species and unsaturated fatty acid sphingomyelin species. Among 96 genes, Lycium Chinense berries up-regulate Glutaredoxin 2 and Ring Finger Protein 7 genes and proteins, able to protect cells from apoptosis. Intrigantly, Lycium Chinense berries down-regulates Prostaglandin H synthase 1 gene but the protein is not expressed in HepG2 cells.

CONCLUSION

The results identify acid sphingomyelinase as a novel target of Lycium Chinense berries to decrease saturated/unsaturated fatty acid sphingomyelin ratio, known to be useful for cell health. Consistent with these data, the berries regulate specifically gene expression to protect cells from apoptosis.

DOI: 10.1186/s12944-016-0351-z
PubMed: 27756324
PubMed Central: PMC5070216

Affiliations:

Italie

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000397
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Le document en format XML

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<author><name sortKey="Beccari, Tommaso" sort="Beccari, Tommaso" uniqKey="Beccari T" first="Tommaso" last="Beccari">Tommaso Beccari</name>
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<series><title level="j">Lipids in health and disease</title>
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<term>Antioxidants (chemistry)</term>
<term>Cell Survival (drug effects)</term>
<term>Fruit (chemistry)</term>
<term>Gene Expression Regulation (drug effects)</term>
<term>Hep G2 Cells (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Lycium (chemistry)</term>
<term>Medicine, Chinese Traditional (MeSH)</term>
<term>Plant Extracts (administration & dosage)</term>
<term>Plant Extracts (chemistry)</term>
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<term>Sphingomyelins (metabolism)</term>
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<term>Antioxydants (composition chimique)</term>
<term>Cellules HepG2 (MeSH)</term>
<term>Extraits de plantes (administration et posologie)</term>
<term>Extraits de plantes (composition chimique)</term>
<term>Fruit (composition chimique)</term>
<term>Humains (MeSH)</term>
<term>Lycium (composition chimique)</term>
<term>Médecine traditionnelle chinoise (MeSH)</term>
<term>Régulation de l'expression des gènes (effets des médicaments et des substances chimiques)</term>
<term>Sphingomyeline phosphodiesterase (biosynthèse)</term>
<term>Sphingomyéline (métabolisme)</term>
<term>Survie cellulaire (effets des médicaments et des substances chimiques)</term>
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<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Antioxidants</term>
<term>Plant Extracts</term>
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<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Sphingomyelin Phosphodiesterase</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antioxidants</term>
<term>Plant Extracts</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Antioxydants</term>
<term>Extraits de plantes</term>
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<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Sphingomyeline phosphodiesterase</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Fruit</term>
<term>Lycium</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Antioxydants</term>
<term>Extraits de plantes</term>
<term>Fruit</term>
<term>Lycium</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Survival</term>
<term>Gene Expression Regulation</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Régulation de l'expression des gènes</term>
<term>Survie cellulaire</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Sphingomyelins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Sphingomyéline</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Hep G2 Cells</term>
<term>Humans</term>
<term>Medicine, Chinese Traditional</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Cellules HepG2</term>
<term>Humains</term>
<term>Médecine traditionnelle chinoise</term>
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<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Sphingomyelin plays very important roles in cell function under physiological and pathological conditions. Physical and chemical stimuli produce reactive oxygen species that stimulate acid sphingomyelinase to induce apoptosis. Antioxidant plants of the traditional Chinese Pharmacopoeia, such as Lycium Barbarum and Lycium Chinense, have become increasingly popular in Western countries. We investigated the effects of Lycium Chinense on acid sphingomyelinase and sphingomyelin species in relation to gene expression.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODS</b>
</p>
<p>We prepared Lycium Chinense berry extracts and evaluated their antioxidant properties. Increasing amount of extracts was used to test cytotoxic and genotoxic effect on HepG2 cells. Gene expression, protein amount and enzyme activity of acid sphingomyelinase were tested by RT-PCR, immunoblotting and enzymatic activity assay, respectively. Sphingomyelin species were analyzed by UFLC MS/MS. A panel of 96 genes involved in oxidative stress, proliferation, apoptosis and cancer was used to test the effect of LC on gene expression. GLRX2, RNF7, and PTGS1 proteins were analyzed by immunoblotting.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>We showed that Lycium Chinense berries have high antioxidant properties, have an IC50value of 9.55 mg/mL, do not induce genotoxic effect and maintain high level of cell viability. The berry extracts inhibit acid sphingomyelinase activity and increase both very long fatty acid sphingomyelin species and unsaturated fatty acid sphingomyelin species. Among 96 genes, Lycium Chinense berries up-regulate Glutaredoxin 2 and Ring Finger Protein 7 genes and proteins, able to protect cells from apoptosis. Intrigantly, Lycium Chinense berries down-regulates Prostaglandin H synthase 1 gene but the protein is not expressed in HepG2 cells.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSION</b>
</p>
<p>The results identify acid sphingomyelinase as a novel target of Lycium Chinense berries to decrease saturated/unsaturated fatty acid sphingomyelin ratio, known to be useful for cell health. Consistent with these data, the berries regulate specifically gene expression to protect cells from apoptosis.</p>
</div>
</front>
</TEI>
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<DateCompleted><Year>2017</Year>
<Month>04</Month>
<Day>04</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1476-511X</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>15</Volume>
<Issue>1</Issue>
<PubDate><Year>2016</Year>
<Month>Oct</Month>
<Day>19</Day>
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<Title>Lipids in health and disease</Title>
<ISOAbbreviation>Lipids Health Dis</ISOAbbreviation>
</Journal>
<ArticleTitle>Acid sphingomyelinase as target of Lycium Chinense: promising new action for cell health.</ArticleTitle>
<Pagination><MedlinePgn>183</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Sphingomyelin plays very important roles in cell function under physiological and pathological conditions. Physical and chemical stimuli produce reactive oxygen species that stimulate acid sphingomyelinase to induce apoptosis. Antioxidant plants of the traditional Chinese Pharmacopoeia, such as Lycium Barbarum and Lycium Chinense, have become increasingly popular in Western countries. We investigated the effects of Lycium Chinense on acid sphingomyelinase and sphingomyelin species in relation to gene expression.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">We prepared Lycium Chinense berry extracts and evaluated their antioxidant properties. Increasing amount of extracts was used to test cytotoxic and genotoxic effect on HepG2 cells. Gene expression, protein amount and enzyme activity of acid sphingomyelinase were tested by RT-PCR, immunoblotting and enzymatic activity assay, respectively. Sphingomyelin species were analyzed by UFLC MS/MS. A panel of 96 genes involved in oxidative stress, proliferation, apoptosis and cancer was used to test the effect of LC on gene expression. GLRX2, RNF7, and PTGS1 proteins were analyzed by immunoblotting.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">We showed that Lycium Chinense berries have high antioxidant properties, have an IC50value of 9.55 mg/mL, do not induce genotoxic effect and maintain high level of cell viability. The berry extracts inhibit acid sphingomyelinase activity and increase both very long fatty acid sphingomyelin species and unsaturated fatty acid sphingomyelin species. Among 96 genes, Lycium Chinense berries up-regulate Glutaredoxin 2 and Ring Finger Protein 7 genes and proteins, able to protect cells from apoptosis. Intrigantly, Lycium Chinense berries down-regulates Prostaglandin H synthase 1 gene but the protein is not expressed in HepG2 cells.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The results identify acid sphingomyelinase as a novel target of Lycium Chinense berries to decrease saturated/unsaturated fatty acid sphingomyelin ratio, known to be useful for cell health. Consistent with these data, the berries regulate specifically gene expression to protect cells from apoptosis.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ceccarini</LastName>
<ForeName>Maria Rachele</ForeName>
<Initials>MR</Initials>
<AffiliationInfo><Affiliation>Department of Pharmaceutical Science, University of Perugia, Via Fabretti 48, 06122, Perugia, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Codini</LastName>
<ForeName>Michela</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Pharmaceutical Science, University of Perugia, Via Fabretti 48, 06122, Perugia, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cataldi</LastName>
<ForeName>Samuela</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Pharmaceutical Science, University of Perugia, Via Fabretti 48, 06122, Perugia, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Vannini</LastName>
<ForeName>Samuele</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Pharmaceutical Science, University of Perugia, Via Fabretti 48, 06122, Perugia, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lazzarini</LastName>
<ForeName>Andrea</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Laboratory of Nuclear Lipid BioPathology, CRABiON, Perugia, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Floridi</LastName>
<ForeName>Alessandro</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Laboratory of Nuclear Lipid BioPathology, CRABiON, Perugia, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Moretti</LastName>
<ForeName>Massimo</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Pharmaceutical Science, University of Perugia, Via Fabretti 48, 06122, Perugia, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Villarini</LastName>
<ForeName>Milena</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Pharmaceutical Science, University of Perugia, Via Fabretti 48, 06122, Perugia, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fioretti</LastName>
<ForeName>Bernard</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Department of Chemistry, Biology and Biotechnology, University of Perugia, Via Elce di Sotto 8, 06123, Perugia, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Beccari</LastName>
<ForeName>Tommaso</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Department of Pharmaceutical Science, University of Perugia, Via Fabretti 48, 06122, Perugia, Italy.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Albi</LastName>
<ForeName>Elisabetta</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Laboratory of Nuclear Lipid BioPathology, CRABiON, Perugia, Italy. elisabetta.albi@gmail.com.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>10</Month>
<Day>19</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Lipids Health Dis</MedlineTA>
<NlmUniqueID>101147696</NlmUniqueID>
<ISSNLinking>1476-511X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000975">Antioxidants</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D013109">Sphingomyelins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.1.4.-</RegistryNumber>
<NameOfSubstance UI="C112980">acid sphingomyelinase-1</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.1.4.12</RegistryNumber>
<NameOfSubstance UI="D013108">Sphingomyelin Phosphodiesterase</NameOfSubstance>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D000975" MajorTopicYN="N">Antioxidants</DescriptorName>
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<MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005638" MajorTopicYN="N">Fruit</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D056945" MajorTopicYN="N">Hep G2 Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032305" MajorTopicYN="N">Lycium</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008516" MajorTopicYN="N">Medicine, Chinese Traditional</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName>
<QualifierName UI="Q000008" MajorTopicYN="N">administration & dosage</QualifierName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013108" MajorTopicYN="N">Sphingomyelin Phosphodiesterase</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013109" MajorTopicYN="N">Sphingomyelins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Antioxidant</Keyword>
<Keyword MajorTopicYN="Y">Fatty acids</Keyword>
<Keyword MajorTopicYN="Y">Lycium chinense</Keyword>
<Keyword MajorTopicYN="Y">Sphingomyelin</Keyword>
<Keyword MajorTopicYN="Y">Sphingomyelinase</Keyword>
</KeywordList>
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<Month>05</Month>
<Day>05</Day>
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<Month>10</Month>
<Day>12</Day>
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<Hour>6</Hour>
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<name sortKey="Cataldi, Samuela" sort="Cataldi, Samuela" uniqKey="Cataldi S" first="Samuela" last="Cataldi">Samuela Cataldi</name>
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Acid sphingomyelinase as target of Lycium Chinense: promising new action for cell health.

Acid sphingomyelinase as target of Lycium Chinense: promising new action for cell health.

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